Fig. 5: Cell localization in perigonadal white adipose tissue in Pdgfrb^GFP reporter mice.

a Barplots of marker gene expression used for cell visualization. Data are presented as mean values +/- SEM. b Immunofluorescence staining of pgWAT from Pdgfrb^GFP report line for CD31 (also known as PECAM1) displaying mural cells across the arteriovenous axis. c Same as in b but with staining for CD31 and PDGFRA. Arrows and arrowheads indicate the position of perivascular and interstitial ASC, respectively. d Same as in b but with staining for CD31 and DPP4. The Arrow and arrowhead indicate the location of interstitial ASC2-population and mesothelial cells, respectively. e Maximum intensity projection of slices with mesothelial DPP4 staining in Pdgfrb^CRE-tdTOM/Pdgfra^H2bGFP reporter mice. Predicted mesothelial nuclei (white circles) were marked based on the DPP4 staining pattern, and Pdgfra^H2bGFP-positive nuclei (cyan circles) were marked dependent on the GFP signal. No overlap of mesothelial nuclei and Pdgfra^H2bGFP-positive nuclei could be observed. GFP Green fluorescent protein, pgWAT perigonadal White adipose tissue and SEM Standard error of the mean.