Fig. 6: Cell localization in perigonadal white adipose tissue in PdgfrbCRE-tdTOM/PdgfraH2bGFP reporter mice. | Nature Communications

Fig. 6: Cell localization in perigonadal white adipose tissue in PdgfrbCRE-tdTOM/PdgfraH2bGFP reporter mice.

From: Adipose stem cells are sexually dimorphic cells with dual roles as preadipocytes and resident fibroblasts

Fig. 6

a pgWAT cryo-section staining and 3D rendering in PdgfrbCRE-dtTOM/PdgfraH2bGFP mice with anti-DPP4 antibody staining for ASC2. b pgWAT whole amount staining and 3D rendering in PdgfrbCRE-tdTOM/PdgfraH2bGFP mice with Pdgfra driven GFP expression marked in cyan color. c Schematic cartoon of our view of the perivascular and vascular cells in pgWAT. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). d Immunofluorescence staining of pgWAT from wilt-type mice for NGFR (encoded by the female ASC specific Ngfr transcript), PDGFRA and CD31 e same as in d but staining for ASC2 marker DPP4 instead of the ASC marker PDGFRA. ASC Adipose stem cells, GFP Green fluorescent protein, and pgWAT perigonadal White adipose tissue.

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