Fig. 7: Characterization of Androgen Response Element (ARE) in the upstream regulatory region of Ptpn22. | Nature Communications

Fig. 7: Characterization of Androgen Response Element (ARE) in the upstream regulatory region of Ptpn22.

From: Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression

Fig. 7

a Normalized fold enrichment of AR based on ChIP-seq experiment in the upstream regulatory region of Ptpn22 including the state of the chromatin and base-wise conservation across 60 vertebrate species. b Schematic diagram of regions tested for AR-mediated Ptpn22 promoter activity, encompassing 1 kb upstream regulatory site and their respective Ptpn22 promoter activity measured by luciferase. Mean ± sem. Data from two independent transfection experiments. Significance was calculated using an unpaired t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. c Precise delineation of ARE within the conserved 56nt sequence. RLU - relative luciferase units. In all expression tests, HEK293T cells were transiently transfected with mouse or human, wild-type or mutant Ptpn22 promoter-luciferase reporter plasmid pGL4.23 and pIRES2-zsGreen-1-AR and incubated in the presence or absence of 0.01uM R1881 for 48 h. pRL-CMV was used to control for transfection efficiency. Firefly luciferase levels were normalized to Renilla luciferase levels. Mean ± sem. Significance was calculated using an unpaired t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. Combined data from 4 experiments.

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