Fig. 3: CiMs exhibit neutrophil-like gene signature.
From: IL36G-producing neutrophil-like monocytes promote cachexia in cancer
a Schematic of sample collection for scRNA-Seq. b t-SNE plot of 5358 single cells distributed using a graph-based clustering algorithm (Cell Ranger). c Pathway enrichment analysis for differentially expressed genes in indicated clusters. d Expression intensity and distribution of Il36g are overlaid on the tSNE plot. e, f Heat map and uniform manifold approximation and projection (UMAP) of genes delineated by ICGS in scRNA-seq data of inflammatory monocytes from Mo-Leukemia mice at the time of cachexia onset. g Frequency of Il36g-expressing CiMs in individual ICGS clusters. h DEGs upregulated in Il36g-expressing CiMs. i GO-term enrichment analysis of DEGs upregulated in Il36g-expressing CiMs. Statistical analyses were performed using Fisher’s exact test (c, e, i) and empirical Bayes moderated t test (h). Source data are provided as a Source Data file.
