Fig. 1: PTPN14 expression is induced in VSMCs during neointimal hyperplasia.

A ApoE^−/− mice were subjected to partial ligation of the left carotid artery (LCA) and fed a Western diet for 4 weeks. Representative images of HE, Oil-red O, and immunofluorescence staining of PTPN14 (red) and α-SMA (green) in LCAs. n = 3. Nuclei was counterstained with DAPI (blue). Scale bar, 500 μm (HE, Oil-red O), and 50 μm (immunofluorescence). L: Lumen, M: Media, I: Intima. B Representative images of HE and immunofluorescence staining from LCAs of C57BL/6 mice at 0, 14, and 21 d after wire injury surgery. PTPN14, red; α-SMA, green; DAPI, blue. Scale bar, 50 μm. C Quantification of PTPN14 in the α-SMA-positive area of the LCAs. The data were presented as the means ± SEM, n = 10 (one-way ANOVA with Bonferroni multiple comparison post-hoc test). D, E Western blots and quantification of the expression of PTPN14, OPN, CNN1, α-SMA, and SM22 in LCAs of C57BL/6 mice at 0, 14, and 21 d post-injury. The protein extracts from 2 mice were pooled into 1 sample. The data were presented as the means ± SEM, n = 6 (one-way ANOVA with Bonferroni multiple comparison post-hoc test). F Representative images of HE and immunofluorescence staining of the human coronary artery. PTPN14, red; α-SMA, green; DAPI, blue. Scale bar, 50 μm. G Quantification of the expression of PTPN14 in α-SMA-positive area of human coronary artery. The data were presented as the means ± SEM, n = 15, 11, 19, respectively (one-way ANOVA with Bonferroni multiple comparison post-hoc test). H The correlation analysis between PTPN14 expression and intima-to-media ratio of human coronary arteries, n = 30 (two-tailed nonparametric Spearman correlation). L: Lumen, M: Media, I: Intima.