Fig. 2: PTPN14 aggravates neointimal hyperplasia in a phosphatase activity-dependent manner.
From: PTPN14 aggravates neointimal hyperplasia via boosting PDGFRβ signaling in smooth muscle cells
C57BL/6 mice subjected to wire injury of left carotid arteries (LCAs) were transfected with AdNC, AdPTPN14, and AdPTPN14C1121S for 14 d. A Representative HE staining images of cross-sections from injured LCAs and contralateral uninjured right carotid arteries (RCAs) of mice 14 d after injury. Scale bar, 50 μm. B Quantification analysis of the ratios of intima to lumen, and the circumference of external elastic lamina (EEL) in the cross-sections from the LCAs at 14 d post-injury. The data were presented as the means ± SEM, n = 10 (one-way ANOVA with Bonferroni multiple comparison post-hoc test). C, D Representative immunofluorescence staining and quantification of the percentage of PCNA-positive cells in cross-sections of LCAs. PCNA, red; α-SMA, green; DAPI, blue. Scale bar, 50 μm. The data were presented as the means ± SEM, n = 10 (one-way ANOVA with Bonferroni multiple comparison post-hoc test). E, F Western blots and quantification of PCNA, OPN, VIM, α-SMA, and SM22 in LCAs. Protein extracts from 2 mice were pooled into 1 sample. The data were presented as the means ± SEM, n = 6 (one-way ANOVA with Bonferroni multiple comparison post-hoc test).
