Fig. 3: PTPN14 promotes PDGF-BB-induced SMC migration, proliferation, and dedifferentiation.
From: PTPN14 aggravates neointimal hyperplasia via boosting PDGFRβ signaling in smooth muscle cells
Human aortic smooth muscle cells (HA-VSMCs) that underwent FBS-free starvation were transfected with AdNC, AdPTPN14, or AdPTPN14C1121S for 48 h. A, B Scratch assays were performed on cells with PDGF-BB (20 ng/mL) for the indicated time points. Representative images and quantification were shown. Scale bar, 20 μm. Data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test). C, D HA-VSMCs treated with PDGF-BB (20 ng/mL) for 6 h were subjected to Transwell assays. Representative images and quantitative analyses were presented. Scale bar, 100 μm. Data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test). E, F HA-VSMCs were treated with PDGF-BB (20 ng/mL) for 24 h. Representative immunofluorescence staining and quantification of Ki67-positive cells were shown. F-actin, red; Ki67, green; DAPI, blue. Scale bar, 10 μm. Data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test). G, H Representative western blots and quantification of the protein levels of Flag-tagged PTPN14, PCNA, OPN, VIM, α-SMA, SM22, and GAPDH in the absence or presence of PDGF-BB (20 ng/mL) treatment. The data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test).
