Fig. 5: PTPN14 controls the output of PDGF-BB signaling in VSMCs.
From: PTPN14 aggravates neointimal hyperplasia via boosting PDGFRβ signaling in smooth muscle cells
Human aortic smooth muscle cells (HA-VSMCs) were transfected with control (siNC) or PTPN14 (siPTPN14) small interfering RNA for 48 h, following the treatment of PDGF-BB (20 ng/mL) or PBS control for 24 h. A Heatmap comparison of differentially expression genes among the indicated treatment groups. B The volcano map showed upregulated or downregulated differentially expresses genes induced by PTPN14 knockdown. C The cytoscape visualization of GO molecular function was performed for differentially expressed genes regulated by PTPN14 knockdown. D The GSEA enrichment profile showed the enrichment of differentially expressed genes stimulated by PDGF-BB (20 ng/mL) in siPTPN14 vs. siNC (two-tailed permutation test). E, F HA-VSMCs were harvested after stimulation with PDGF-BB (10 ng/mL) for 0, 10, and 30 min. Western blots and quantification of the indicated proteins were performed. The data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test). G, H Representative western blots and quantification of the indicated protein levels in the presence or absence of PDGF-BB (20 ng/mL) treatment for 24 h. The data were presented as the means ± SEM, n = 6 (two-way ANOVA with Bonferroni multiple comparison post-hoc test).
