Fig. 3: Repeated CBD treatment (100 mg/kg) reduces neuroinflammation in the GPe of a LS mouse model.
From: Cannabidiol ameliorates mitochondrial disease via PPARγ activation in preclinical models
Representative immunofluorescence images (left) and z-stack of higher magnification images (right) (a) and quantification of GFAP fluorescence signal intensity (b) of the astroglial marker GFAP in the GPe of daily CBD- or vehicle-treated control (Gad2:Ndufs4cCT) and Gad2:Ndufs4cKO mice (n = 9 [control-vehicle], 7 [control-CBD], 8 [Gad2:Ndufs4cKO-treated groups], two-way ANOVA/Tukey’s multiple comparisons test, ****p < 0.0001 [genotype effect]; ###p = 0.0008 [treatment effect]). Representative immunofluorescence images (left) and z-stack of higher magnification images (right) (c) and quantification of IBA1 fluorescence signal intensity (d) of the microglia/macrophage marker IBA1 in the GPe of daily CBD- or vehicle-treated Gad2:Ndufs4cCT and Gad2:Ndufs4cKO mice (n = 9 [control-vehicle], 7 [control-CBD], 8 [Gad2:Ndufs4cKO-treated groups], two-way ANOVA/Tukey’s multiple comparisons test, ***p = 0.0002 [genotype effect]; ###p = 0.0002 [treatment effect]). e Quantification of TMEM119 fluorescence signal intensity in the GPe of daily CBD- or vehicle-treated control and Gad2:Ndufs4cKO mice (n = 9 [control-vehicle], 7 [control-CBD], 8 [Gad2:Ndufs4cKO-treated groups], two-way ANOVA/Tukey’s multiple comparisons test, *p = 0.0446 [genotype effect]; ###p = 0.0006 [treatment effect]). f Representative 3D microglia reconstruction using Imaris of each group. g Quantification of soma volume/cell of IBA1+ cells (n = 9 [control-vehicle], 7 [control-CBD and Gad2:Ndufs4cKO-vehicle], 8 [Gad2:Ndufs4cKO-CBD], two-way ANOVA/Tukey’s multiple comparisons test, ****p < 0.0001 [genotype effect]; ####p < 0.0001 [treatment effect]). h Distribution plot of microglial ramification (n = 9 [control-vehicle], 7 [control-CBD and Gad2:Ndufs4cKO-vehicle], 8 [Gad2:Ndufs4cKO-CBD], two-way ANOVA/Tukey’s multiple comparisons test, ****p < 0.0001 [genotype effect]; #p = 0.0477, ###p = 0.0001 [treatment effect]). i Quantification of total dendrite volume/cell of IBA1+ cells (n = 9 [control-vehicle], 7 [control-CBD and Gad2:Ndufs4cKO-vehicle], 8 [Gad2:Ndufs4cKO-CBD], two-way ANOVA/Tukey’s multiple comparisons test, **p = 0.0013 [genotype effect]). j Quantification of total dendrite length/cell of IBA1+ cells (n = 9 [control-vehicle], 7 [control-CBD and Gad2:Ndufs4cKO-vehicle], 8 [Gad2:Ndufs4cKO-CBD]). All data are presented as mean values ± SEM. All source data are provided as a source data file. Veh vehicle, CT control mice, cKO Gad2:Ndufs4cKO mice. Scale bars: a, c: 100 μm (left) and 20 μm (right), f: 8 μm.
