Fig. 7: 4D4 TCR⁺ T cells develop in vivo in a retrogenic mouse model.

A Flow cytometry analysis of bone marrow (BM), thymus (Thy), spleen (Sp), lymph node (LN), liver, lung, and blood of 4D4 retrogenic mice (Rg) or wild type (WT) C57BL/6. Representative plots of GFP and TCRβ expression on 7AAD⁻ CD19⁻ CD11b⁻ cells amongst CD45.2⁺ (from donor transduced BM) or CD45.1⁺ (recipient) in Rg mice or CD45.2⁺ from WT mice. B CD4 and CD8 co-receptor expression and C SAV-PE binding among CD45.2⁺ TCRβ ⁺ GFP⁺ Rg cells in comparison to CD45.1⁺ TCRβ⁺ GFP⁻ cells from the same Rg mice, or CD45.2⁺ TCRβ⁺ GFP⁻ cells from WT mice. Data in (B) are from one Rg mouse, representative of n = 12 Rg mice (acquired over 5 independent experiments for thymus and spleen, or 3 experiments for blood), n = 10 Rg mice for liver and BM (4 experiments), n = 7 Rg mice for lung and LN (3 experiments); and from one WT mouse representative of n = 7 WT mice (4 experiments) for spleen and thymus, n = 6 WT mice for lung, LN and BM (3 experiments), n = 4 WT mice for liver (3 experiments). Data in (C) are from one Rg and from one WT mice representative of 2 experiments with n = 2 or n = 3 Rg and WT organs, with the exception of the liver which is from 1 experiment with n = 3 Rg or n = 3 WT mice. Graphs showing assay variation for (B) and (C) are in Supplementary Fig. 10A and 10B. D Paired TCR analysis of single cells sorted as TCRβ⁺ GFP⁺ or TCRβ⁺ GFP⁻ from the spleen of a retrogenic mouse. Nomenclature as per the IMGT database. Source data are provided as a Source Data file.