Fig. 2: The relative proportion of neurons and glia vary among brain regions. | Nature Communications

Fig. 2: The relative proportion of neurons and glia vary among brain regions.

From: Base excision repair and double strand break repair cooperate to modulate the formation of unrepaired double strand breaks in mouse brain

Fig. 2

Mouse Allen Mouse Brain Atlas map46 highlighting coronal brain sections of the CBL (a) or the STR (f) (Allen Mouse Brain Atlas; mouse.brain-map.org/shown/2011 coronal reference atlas) (http://atlas.brain-map.org/atlas?atlas=1). The anatomical annotations from the Allen Mouse Brain Atlas for STR and CBL were used at the same slice positions. IF staining of neurons and glial cells from coronal brain sections of male (7 weeks) C57BL/6J mice in the CBL (b–d) or the caudoputamen of the STR (g–i). Neurons are co-labeled with an anti-NeuN antibody (green) and DAPI (blue) and appear as teal. Glia stain only with DAPI and appear blue. Red boxes illustrate a segment of the coronal brain section that was magnified in the image below it; scale bars are 1 mm (b, g), 100 µm (c, h), and 10 µm (d, i), as indicated. e The proportion of each cell type in n = 50 cells were assessed in each region from three random tissue sections (n = 3). The data were plotted as % of total cells counted per region in (n = 3) animals. The total % neuronal (N, green) and % glial (G, blue) cell content was visualized to directly compare the CBL and STR. Bars are standard error of the mean (SEM). Source data are provided in Source data file. Regional cell type comparison was determined by a one-way ANOVA, ****P < 0.0001.

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