Fig. 3: Expression of the DNA repair machinery in the mouse brain is region and cell type specific. | Nature Communications

Fig. 3: Expression of the DNA repair machinery in the mouse brain is region and cell type specific.

From: Base excision repair and double strand break repair cooperate to modulate the formation of unrepaired double strand breaks in mouse brain

Fig. 3

a The quadrant of cell images shows expression of two representative cells for DNA repair proteins of five pathway in NeuN (+) (neurons) and NeuN (−) cells (glia) ± DAPI from cortex of 7 weeks male mice, as indicated. Scale bar is 10 µm. Three additional images for protein expression of each DNA repair protein in NeuN (+) (neurons) and NeuN (−) cells (glia) ± DAPI are included in Supplementary Fig. 2. The nuclear contours from the DAPI stain outlined in light blue indicate the position of the nucleus and highlights the poor protein staining intensity in glia. b, c The average per cell expression was quantified from IF intensity for each protein in 50 neurons and 50 glia randomly selected in tissue sections from four brain regions: (CBL (blue), STR (green), CTX (pink), HIP (orange) (n = 3 animals). The repair proteins quantified were APE1, XPA, MRE11, Ku70, and MSH6, as indicated. Data are displayed as a box and whisker plot, where a box indicating the 25th to 75th percentile values, the line indicates the median, with the lower whiskers representing the minimum 25% of data values and the whiskers above the box representing the 25% maximum values. Statistical probability of variance (P) was calculated from n = 50 cells of each type (Neuron (Neu), and (GL) Glia) using two-tailed homoscedastic t-test. Statistical significance among regional expression comparisons is tabulated in Supplementary Table 1. *P = < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.00005.

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