Fig. 2: Three lipids binding in a channel in MtEfpA. | Nature Communications

Fig. 2: Three lipids binding in a channel in MtEfpA.

From: Structures of the Mycobacterium tuberculosis efflux pump EfpA reveal the mechanisms of transport and inhibition

Fig. 2

a The model of MtEfpA with densities of cardiolipin (CDL, green) and 2 molecules of phosphatidylethanolamine (PE, colored wheat at site B and yellow at site C). b Superimposition of the MtEfpA model and the cardiolipin density (5σ) at site A, shown in side view (upper) and top view (bottom). The gray dashed lines represent the missing ends of two tails extending inward toward the membrane. c The surface and residues at the lateral gate of cardiolipin insert into MtEfpA. d The residues of MtEfpA that interact with cardiolipin within 4 Å. e The two lateral gates of a channel in MtEfpA open separately towards the inner and outer leaflets of the membrane. The dashed line represents the boundary between the inner leaflet and outer leaflet of membrane. f Three lipids bind to MtEfpA in “staircase”-shaped tunnel from a side view. g Cross-sectional perspectives of the lipids-binding channel in MtEfpA. Left, view from side. Right, view from top. The two lateral gates are indicated by black and red stars, while the funnel opens toward the periplasm, marked with a black triangle. The cartoon on top represents the cross-sectional position from a lateral perspective.

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