Fig. 1: Overall structures of the human VMAT2.
From: Neurotransmitter recognition by human vesicular monoamine transporter 2
a Mechanism of monoamine uptake into vesicles by VMAT2. b Cryo-EM density of VMAT2apo-Fab0801 complex at a threshold level of 1.2. c Dopamine transport activity of wild-type VMAT2 (VMAT2WT) and cryo-EM construct (VMAT2Cryo) in the presence or absence of the intracellular recognition antibody Fab0801. Proteoliposomes containing purified VMAT2 protein were prepared in buffer at pH 6.0, incubated in buffer at either pH 6.0 (−) or pH 7.5 (+) for 1 min. Error bars represent mean ± SEM; points represent biologically independent measurements (n = 6 for VMAT2WT; n = 5 for others). d Cartoon representation of VMAT2apo. e, f Electrostatic surface representation of VMAT2apo highlighting the central cavity. Side view (e) and top view (f).
