Fig. 4: CARNMT1 is responsible for the His methylation of ZF proteins. | Nature Communications

Fig. 4: CARNMT1 is responsible for the His methylation of ZF proteins.

From: Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers

Fig. 4

a The interaction between CARNMT1 and ZF proteins was confirmed by the co-purification of the HA tagged CARNMT1 with the Flag tagged ZF proteins. 2 times each experiment was repeated independently with similar results. b Deletion of CARNMT1 in HeLa cells. 2 times each experiment was repeated independently with similar results. c His methylation of over-expressed U2AF1 cannot be observed after the deletion of CARNMT1. d Supplementation of wild-type and mutated CARNMT1 in CARNMT1-KO cells. 2 times each experiment was repeated independently with similar results. e The methylation of over-expressed U2AF1 in CARNMT1 KO cells can be recovered by the supplementation of WT CARNMT1 and was not recovered by the supplementation of mutated CARNMT1. f ZF domain peptides corresponding to ZF1 of U2AF1 can be methylated by CARNMT1 in the in-vitro methylation assay. g ZF1 in the purified U2AF1 from E.coli can be methylated by CARNMT1 in the in-vitro methylation assay, while the ZF2 cannot be modified. The retention time information can be found in the identification result in Supplementary Table 8.

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