Fig. 4: Pro-efferocytic therapies induce favorable immunological gene expression changes across species. | Nature Communications

Fig. 4: Pro-efferocytic therapies induce favorable immunological gene expression changes across species.

From: Pro-efferocytic nanotherapies reduce vascular inflammation without inducing anemia in a large animal model of atherosclerosis

Fig. 4

a Bulk porcine RNA-sequencing experimental workflow. b Volcano plot of differentially expressed porcine genes from bulk RNA-sequencing in SWNT-SHP1i (n = 3) versus SWNT control (n = 3) common carotid arteries. Red and blue points represent up- and downregulated transcripts (log2 fold change >1 or <−1), respectively. Orange points indicate significantly differentially expressed genes, selected by log2 fold change (>2 or <−2) and adjusted p-value (<0.05, Benjamin–Hochberg correction). Gray points represent nonsignificant genes. Statistical significance was assessed using a likelihood ratio test (LRT) with two-sided testing. c Venn Diagram representing overlap of 35 significant differentially expressed genes between pig bulk RNA-sequencing results and published mouse scRNA-sequencing data from monocytes and macrophages. d Biological Process GO enrichment and pathway analysis of the 35 DEGs shared across species reveal pathways governing immune system response, response to an external biotic stimulus, endocytosis, and phagocytosis in the SWNT-SHP1i group. Adjusted p-value derived from the Benjamini & Hochberg test. Figure 4/panel a created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).

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