Fig. 3: Negligible immune response was elicited by the EVADE material implanted for one month.
From: Immunocompatible elastomer with increased resistance to the foreign body response
Discs made of PDMS and H90 with a diameter of 4 mm and a thickness of 1 mm were implanted SubQ on mouse backs. The tissue surrounding the implant was analyzed 30 days post-implantation. Scale bar = 25 μm. a Immunofluorescence staining of sections for α-SMA (red, fibroblasts), CD3 (green, T cells), F4/80 (purple, macrophages), and nuclei stained via DAPI (blue) (n = 3 mice per group). b, c Gene expression changes of PDMS/mock comparison (b) and H90/mock comparison (c). d, e KEGG pathway enrichment analysis of PDMS/mock comparison (d) and H90/mock comparison (e). f Gene expression analysis of phenotypic markers in tissues surrounding the H90 and PDMS materials, with data normalized to the mock group and presented on a log2 scale (n = 3 mice per group). g Semiquantitative western blot analysis of inflammatory and FBR-related factor expression in tissues surrounding the H90 and PDMS materials (n = 3 mice per group). h qRT-PCR analysis of mRNA expression for FBR-related genes (n = 5 mice per group, mean ± s.d.). Statistical significance was determined by one-way analysis of variance (ANOVA) with Bonferroni multiple comparison test.
