Fig. 5: Global Ca²⁺ responses evoked by MASTER-NAADP in Jurkat T cells.

Global Ca²⁺ signaling was analyzed in Jurkat WT T cells (A) and Jurkat Hn1l/Jpt2^-/- T cells (B), loaded with Fura2-AM. MASTER-NAADP (100 nM), MASTER-NADP (100 nM), or NAADP-AM (100 nM) were added as indicated. Experiments were carried out at 37 °C and SOCE was blocked by pre-incubation of 50 µM Synta66 for 5 min prior to imaging. Number of cells indicated in figure. Aggregated data are mean amplitude per peak (C), mean number of Ca²⁺ peaks (D), percentage of responding cells (E), and calculation of the mean responsiveness (number of peaks * amplitude; F presented as mean ± SEM; Jurkat WT MASTER-NAADP, n = 240 cells; Jurkat WT MASTER-NADP, n = 120 cells; Jurkat WT NAADP-AM, n = 91 cells; Jurkat Hn1l/Jpt2^-/- MASTER-NAADP, n = 181 cells; Jurkat Hn1l/Jpt2^-/- MASTER-NADP, n = 113 cells; Jurkat Hn1l/Jpt2^-/- NAADP-AM, n = 110 cells. Nonparametric Kruskal-Wallis test and Dunn’s correction for multiple testing *P < 0.05; ****P < 0.0001. Source data and exact p values are provided as a Source Data file.