Fig. 6: Global Ca2+ responses evoked by MASTER-NAADP in KHYG-1 NK cells and Neuro2A cells.
From: MASTER-NAADP: a membrane permeable precursor of the Ca2+ mobilizing second messenger NAADP
Global Ca2+ signaling was analyzed in Fura2-loaded KHYG-1 cells (A–E), Neuro2A WT or Neuro2A Hn1l/Jpt2-/- cells (F–K). A MASTER-NAADP (100 µM) or MASTER-NADP (100 µM) were added at RT as indicated. Number of cells indicated in figure. Aggregated data are mean amplitude per peak (B), mean number of Ca2+ peaks (C), responding cells (D), and calculation of the mean responsiveness (number of peaks * amplitude; E; as mean ± SEM; MASTER-NAADP, n = 339 cells; MASTER-NADP, n = 425 cells; two-sided, nonparametric Mann-Whitney U test: ****P < 0.0001. F and G MASTER-NAADP (100 µM) or MASTER-NADP (100 µM) were added at RT as indicated to Neuro2A WT (F) or Neuro2A Hn1l/Jpt2-/- cells (G). Number of cells is indicated in figure. Aggregated data are mean amplitude per peak (H), mean number of Ca2+ peaks (I), responding cells (J), and calculation of the mean responsiveness (number of peaks * amplitude; K; as means ± SEM; Neuro2A WT MASTER-NAADP, n = 71 cells; Neuro2A WT MASTER-NADP, n = 50 cells; Neuro2A Hn1l/Jpt2-/- clone 1G4 MASTER-NAADP, n = 50 cells; Neuro2A Hn1l/Jpt2-/- clone 1G4 MASTER-NADP, n = 39 cells; Nonparametric Kruskal-Wallis test and Dunn’s correction for multiple testing ****P < 0.0001. Source data are and exact p values provided as a Source Data file.
