Fig. 2: Shape variation of sexual and asexual triploid females.

a Landmarks position. Red dots and blue lines represent the landmarks (1–9, numbered in the white circles) and the semi-landmarks, respectively, used in this study. For the semi-landmarks, 15 were used on the chin curve and 25 on the operculum curve. b, c Shape variation. Principal Component Analysis (PCA) of the two hybrid lineages (A-11 and B-01, shape) with individuals originating from two natural habitats (creek and lake, respectively) (b) or from common garden conditions (c), and according to their reproductive strategy (color). In both (b) and (c), small and semitransparent data points represent individuals and plain and large data points with error bars represent group (reproduction mode and lineage) centroids and their standard deviation. Error bars were computed from all individuals within a given lineage, reproduction mode, and environmental condition: (b) A-11/Asexual: n = 9; A-11/Sexual: n = 14; B-01/Asexual: n = 26; B-01/Sexual: n = 33 and (c) A-11/Asexual: n = 8; A-11/Sexual: n = 4; B-01/Asexual: n = 5; B-01/Sexual: n = 4 (Supplementary Table II). While a single PCA was performed among all samples, two panels according to environments were used to represent the ordination, for more clarity. Convex hulls and extreme shapes of the PCA are shown in Supplementary Fig. 1. Source data for this figure can be found in Supplementary Data 1 and Supplementary Code 1.