Fig. 2: Mapping of RVFV nucleoprotein-RNA interaction by iCLIP2 in virus particles. | Nature Communications

Fig. 2: Mapping of RVFV nucleoprotein-RNA interaction by iCLIP2 in virus particles.

From: Nucleocapsids of the Rift Valley fever virus ambisense S segment contain an exposed RNA element in the center that overlaps with the intergenic region

Fig. 2

Integrative Genomics Viewer (IGV) tracks representing the number of N-RNA crosslink reads (Y-axis) at single nucleotide positions (X-axis) along the RVFV MP-12 segment RNA sequences, as determined by N protein UV crosslinking and immunoprecipitation (iCLIP2) of virion ribonucleoprotein particles (RNPs). For each viral segment (L, M, or S, as schematically indicated on the left), viral RNA in genomic-sense orientation is shown 3′–5′ (vRNA) and 5′–3′ in antigenomic-sense orientation (cRNA). Two different monoclonal anti-N antibodies were used for iCLIP2 experiments and data corresponding to each antibody are shown (“Epitope 1”, “Epitope 2”), with three biological replicates in each case (“R1-R3”). Note that all panels were adjusted to the same width for visualization of good reproducibility of all six iCLIP2 patterns, although the segment lengths vary between 6404 (L), 3885 (M), and 1690 (S) nt. Track height range (crosslink site count) is adjusted by autoscale and presented on the right-hand side of each track. In this and subsequent iCLIP2 representations, reads from the 3′ and 5′ ends are shown out of range to obtain optimal resolution of the central parts of the sequences.

Back to article page