Fig. 1: Overexpression of SLC7A14 in POMC neurons is resistant to reduction in lipolysis with age.
From: Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice
a SLC7A14 and TUBULIN proteins in hypothalamus. b Immunofluorescence (IF) staining for SLC7A14 ARC sections (left), and quantification of SLC7A14 cell numbers (right). c, d IF staining for tdTomato, SLC7A14 and merge in ARC sections (left), and quantification of SLC7A14 and tdTomato colocalized cell numbers (right). e Fat mass by NMR. f Lean mass by NMR. g Tissue weight. h Representative images of hematoxylin and eosin (H&E) staining of sWAT. i Cell size of sWAT quantified by Image J analysis of H&E images. j The DNA content of sWAT. k P-HSL, t-HSL, p-PKA substrates, FAS, CPT1α, CD36 and TUBULIN proteins in sWAT by western blotting (left) and quantified by densitometric analysis (right). n = 6 mice. l Glycerol release assays in sWAT. m The cAMP levels of sWAT. Studies for a–c were conducted using 2-month-old mice (2 M) or 22-month-old mice (22 M); d–m were conducted using 20-month-old POMC Cre mice receiving AAVs expressing mCherry (- AAV-SLC7A14) or SLC7A14 (+AAV-SLC7A14) in ARC. Metabolic tests were performed 1 month after adeno-associated virus injection. For (a), n = 6. For (b), n = 6 (2 M) and 5 (22 M). For (c), n = 4 (2 M) and 5 (22 M). For (d–j and m), n = 5. For k, n = 6. For l, n = 6 (-AAV-SLC7A14) and 5 (+AAV-SLC7A14). Data are expressed as the mean ± SEM, with individual data points. Data were analyzed by two-tailed unpaired Student’s t-test (a–g, i–m). Source data are provided as a Source Data file.
