Fig. 2: The absence of SLC7A14 in POMC neurons mimic age-induced lipolysis impairment.
From: Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice
a IF staining for tdTomato, SLC7A14 and merge in ARC sections (left), and quantification of SLC7A14 and tdTomato colocalized cell numbers (right). b Fat mass by NMR. c Tissue weight. d Representative images of H&E staining of sWAT. e Cell size of sWAT quantified by Image J analysis of H&E images. f The DNA content of sWAT. g P-HSL, t-HSL, p-PKA substrates, FAS, CPT1α, CD36 and TUBULIN proteins in sWAT by western blotting (left) and quantified by densitometric analysis (right). h Glycerol release assays in sWAT. i The cAMP levels of sWAT. Studies for (a–i) were conducted using control mice (- SPKO) or mice with SLC7A14 deletion in POMC neurons (+ SPKO), and at age of 6-month-old in (a), (c–i). For (a and h), n = 5. For (b), n = 5 (- SPKO) and 6 (+SPKO). For (c), n = 7 (- SPKO) and 6 (+SPKO). For (d, e, g and i), n = 6. For (e), n = 6. For (f), n = 7 (- SPKO) and 6 (+SPKO). Data are expressed as the mean ± SEM, with individual data points. Data were analyzed by two-tailed unpaired Student’s t-test (a, c, e–i), or two-way RM ANOVA with Geisser-Greenhouse’s correction (b). Source data are provided as a Source Data file.
