Fig. 3: The decreased TCDCA mediates SPKO-induced WAT lipolysis impairment.
From: Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice
a Metabolite set enrichment analysis. b–d The TCDCA levels of serum. e The TCDCA levels of WAT. f Fat mass by NMR. g Tissue weight. h Representative images of H&E staining of sWAT. i Cell size of sWAT quantified by Image J analysis of H&E images. j P-HSL and t-HSL proteins in sWAT. k Glycerol release assays in sWAT. l The cAMP levels of sWAT. Studies for a and b were conducted using 20-month-old POMC Cre mice receiving AAVs expressing mCherry (-AAV-SLC7A14) or SLC7A14 (+AAV-SLC7A14) in ARC. c–l were conducted using 5-month-old control mice (-SPKO) or mice with SLC7A14 deletion in POMC neurons (+ SPKO). Mice were intraperitoneally (i.p.) injected with PBS (-TCDCA) or 50 mg/kg body weight TCDCA (+TCDCA) every other day. On the fifteenth day, mice were i.p. injected with a single dose of TCDCA before euthanized in (d–l). For (a, b), n = 5 (- AAV-SLC7A14) and 4 (+AAV-SLC7A14). For (c), n = 7 (- SPKO) and 4 (+SPKO). For (d), n = 6 (- SPKO - TCDCA), 5 (+SPKO - TCDCA), 7 (- SPKO + TCDCA) and 7 (+SPKO + TCDCA). For (e), n = 6 (- SPKO - TCDCA), 7 (+SPKO - TCDCA), 7 (- SPKO + TCDCA) and 6 (+SPKO + TCDCA). For (f and h–j), n = 5. For (g), n = 6. For k, n = 5 (−SPKO - TCDCA), 5 (+SPKO - TCDCA), 5 (−SPKO + TCDCA) and 6 (+SPKO + TCDCA). For l, n = 5 (−SPKO - TCDCA), 6 (+SPKO - TCDCA), 6 (- SPKO + TCDCA) and 5 (+SPKO + TCDCA). Data are expressed as the mean ± SEM, with individual data points. Data were analyzed by Fisher’s exact test (a) or two-tailed unpaired Student’s t-test (b, c), or ordinary two-way ANOVA with Tukey’s multiple comparisons test (d–g, i–l). Source data are provided as a Source Data file.
