Fig. 5: The intestinal ASBT mediates POMC SLC7A14-induced TCDCA levels and WAT lipolysis.
From: Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice
a and e The TCDCA levels of ileum. b and f The TCDCA levels of feces. c, g, o and t The mRNA levels of Asbt in ileum by RT-PCR. d and h ASBT and TUBULIN proteins in ileum. i SLC7A14 and TUBULIN proteins in ARC. j The TCDCA levels of serum. k P-HSL and t-HSL proteins in sWAT. l Glycerol release assays in sWAT. m and s The Pparα mRNA levels in ileum by RT-PCR. n PPARα and TUBULIN proteins in ileum. p–r The OEA levels of ileum. Studies for a-d, q and i-o were conducted using POMC Cre mice receiving AAVs expressing mCherry (- AAV-SLC7A14) or SLC7A14 (+AAV-SLC7A14) in ARC, and treated twice daily with control vehicle (- linerixibat) or 0.05 mg/kg linerixibat (+ linerixibat) via oral gavage in i–l, or the ileum of mice was injected with AAVs expressing shPPARα (+ AAV-shPPARα) or EGFP (−AAV-shPPARα) in m–o. e–h, p and r–t were conducted using 6-month-old control mice (- SPKO) or mice with SLC7A14 deletion in POMC neurons (+ SPKO), and mice were given OEA (10 mg/kg, aladdin) in (r–t). For a, n = 7 (- AAV-SLC7A14) and 6 (+AAV-SLC7A14). For b, n = 6 (- AAV-SLC7A14) and 5 (+AAV-SLC7A14). For (c), n = 7. For (d, i–k, n, and p–t), n = 5. For e and g, n = 5 (- SPKO) and 6 (+SPKO). For (f), n = 6 (- SPKO) and 5 (+SPKO). For (h) and m–o, n = 6. For l, n = 5 (- AAV-SLC7A14 - Linerixibat), 5 (+AAV-SLC7A14 - Linerixibat), 6 (- AAV-SLC7A14 + Linerixibat) and 5 (+AAV-SLC7A14 + Linerixibat). Data are expressed as the mean ± SEM, with individual data points. Data were analyzed by two-tailed unpaired Student’s t-test (a–h, p, q), or ordinary two-way ANOVA with Tukey’s multiple comparisons test (i–o, r–t). Source data are provided as a Source Data file.
