Fig. 6: POMC SLC7A14 regulates ASBT expression via intestinal sympathetic afferent nerves.
From: Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice
a Representative micrographs of the ileum wall demonstrating neurons expressing PRV. b Representative micrographs of the CG-SMG demonstrating neurons expressing PRV. c Representative micrographs of the brain demonstrating neurons expressing PRV, tdTomato and merge. d–g, k TH and TUBULIN proteins in ileum. e–h IF staining for DAPI, TH and merge in ileum. (f, i and l) The NE levels of ileum. j IF staining for tdTomato, SLC7A14 and merge in ARC. m The OEA levels of ileum. n The Pparα mRNA levels in ileum. o The Asbt mRNA levels in ileum. p ASBT and TUBULIN proteins in ileum. q The TCDCA levels of serum. Studies for a-c were conducted using 5-month-old POMC Cre mice, which carrying Ai9 (tdTomato) reporter, injected with pseudorabies virus (BC-PRV-531-PRV-CAG-EGFP) for 6 days in ileum. d–f were conducted using 5-month-old control mice (- SPKO) or mice with SLC7A14 deletion in POMC neurons (+ SPKO); (g–i, j–q) were conducted using 20 month-old POMC Cre mice receiving AAVs expressing mCherry (- AAV-SLC7A14) or SLC7A14 (+AAV-SLC7A14) in ARC, and treated with sham surgery (- CG/SMG ganglionectomy) or surgical removal of celiac-superior mesenteric ganglion (+ CG/SMG ganglionectomy) in j-q. For a–c, n = 7. For (d–g, n) n = 6. For (e) and (h) n = 3. For (f–i, k–m) and (p-q) n = 5. For (j) n = 4 (−AAV-SLC7A14 - CG/SMG ganglionectomy), 4 (+AAV-SLC7A14 - CG/SMG ganglionectomy), 5 (- AAV-SLC7A14 + CG/SMG ganglionectomy) and 5 (+AV-SLC7A14 + CG/SMG ganglionectomy). For (o) n = 6 (−AAV-SLC7A14 - CG/SMG ganglionectomy), 6 (+AAV-SLC7A14 - CG/SMG ganglionectomy), 7 (−AAV-SLC7A14 + CG/SMG ganglionectomy) and 5 (+AAV-SLC7A14 + CG/SMG ganglionectomy). Data are expressed as the mean ± SEM, with individual data points. Data were analyzed by two-tailed unpaired Student’s t-test (d–f, g–i) or ordinary two-way ANOVA with Tukey’s multiple comparisons test (j–q). Source data are provided as a Source Data file.
