Fig. 2: Heterogeneity of myeloid and lymphoid cell populations within adipocytic tumors.
From: Cellular origin and clonal evolution of human dedifferentiated liposarcoma
A UMAP of the 10 main myeloid cell types identified according to the expression of marker genes. B Dot plots showing the expression of 22 marker genes across the clusters (n = 26,557 single cells). C Proportion of the myeloid populations among all myeloid cells according to histological subtypes (Lipoma, N = 1; WDLPS, N = 5; DDLPS, N = 19; DDLPS-WD, N = 10; DDLPS-DD, N = 9). D Proportion of CD163-positive myeloid cells by immunohistochemistry (IHC) in the independent cohort of paired WD and DD DDLPS (N = 15). The mean value is indicated in bold ( ± SD). p-val = 2.6 × 10−6 (two-sided t test). E In situ multiplex immunofluorescence (mIF) staining of tumor cells (MDM2+), monocytes/macrophages (CD163+), M2-like macrophages (TAM2-like, CD163+ and CD68+), myeloid-derived suppressor cells (MDSC, CD33+) and tumor-associated neutrophils (TAN, S100A8+) (representative pictures from one paired tumor sample, n = 7 paired samples analyzed); scale bar: 50 µm. F UMAP of the 8 main lymphoid cell types. G Dot plots showing the expression of 10 marker genes across the clusters (n = 19,978 single cells). H Proportions of the lymphoid populations among all lymphoid cells according to histological subtypes (Lipoma, N = 1; WDLPS, N = 5; DDLPS, N = 19; DDLPS-WD, N = 10; DDLPS-DD, N = 9). I Proportion of CD3-positive (left panel, N = 21 DDLPS-WD and N = 19 DDLPS-DD) and FOXP3-positive (right panel, N = 15 DDLPS-WD and N = 10 DDLPS-DD) T cells by IHC in an independent cohort of WD and DD DDLPS components; the number of CD3 and FOXP3-positive cells was normalized on the same number of total cells in both components. The mean value is indicated in bold (± SD). p-val = 1 × 10−3 (CD3) and p-val = 4 × 10−4 (FOXP3) (two-sided t test). J mIF staining of tumor cells (MDM2+), T lymphocytes (CD4+ or CD8+), CD4 Treg cells (CD4+, FOXP3+), and exhausted CD8 T cells (CD8+, PD1+) (representative pictures from one paired tumor sample, n = 7 paired samples analyzed); scale bar: 50 µm. Abbreviations: c: classical; i: intermediate; nc: non-classical. ***: p-val ≤ 0.001. Source data are provided as a Source Data file.
