Fig. 5: Intranasal treatment with GS-441524 of intranasally inoculated AG129 mice reduces viral loads in the lung and NPLT-washes and lung pathology. | Nature Communications

Fig. 5: Intranasal treatment with GS-441524 of intranasally inoculated AG129 mice reduces viral loads in the lung and NPLT-washes and lung pathology.

From: A robust mouse model of HPIV-3 infection and efficacy of GS-441524 against virus-induced lung pathology

Fig. 5

a Setup of the study. AG129 mice were intranasally treated once daily with either vehicle or GS-441524 (40 µL/mouse of a 30 mg/mL solution) from day −1 p.i. to day 2 p.i., and intranasally inoculated with 1.5 × 106 TCID50 of HPIV-3 at day 0 p.i. Lung samples and NPLT-washes were collected at day 3 p.i. to measure viral loads. b Infectious virus titers are expressed as log10 TCID50 per milligram of lung sample. c HPIV-3 RNA levels are expressed as log10 TCID50 equivalent per milligram of lung sample. d Infectious virus titers are expressed as log10 TCID50 per milliliter of NPLT-wash. e HPIV-3 RNA levels are expressed as log10 TCID50 equivalent per milliliter of NPLT-wash. b–e LLOD presents the lower limit of detection. LLOQ presents the lower limit of quantification. Data are from two independent experiments with ten mice per group. Data were analyzed by the two-tailed Mann–Whitney U test. *p = 0.0312, **p = 0.0071, ****p < 0.0001. f Setup of the study. AG129 mice were intranasally treated once daily with either vehicle or GS-441524 (40 µL/mouse of a 30 mg/mL solution) starting from day −1 p.i. and continued for five consecutive days. The mice were intranasally inoculated with 1.5 × 106 TCID50 of HPIV-3 at day 0 p.i. Lung samples were collected at day 6 p.i. for lung histopathology assessment. g Weight change at day 6 p.i. is presented as a percentage of the body weight at day −1 p.i., when treatment was started. h Representative H&E-stained images of the lung show bronchopneumonia (green arrow), perivascular inflammation (red arrows), peribronchial inflammation (blue arrow), and pneumocyte hyperplasia (orange arrow). The scale bar is 50 µm. i Cumulative severity scores of lungs of all infected mice. g, i Data are from one experiment, with seven mice per group. Data were analyzed with the two-tailed Mann–Whitney U test. ns nonsignificant, **p = 0.0070. Individual data and median values (indicated by bars) are presented in all graphs. Source data are provided as a Source Data file. a, f were designed with BioRender.

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