Fig. 5: TauC3 co-accumulates with Serine 416 phosphorylation in Alzheimer’s disease patient brains.
From: Phosphorylation of tau at a single residue inhibits binding to the E3 ubiquitin ligase, CHIP
A Representative micrographs of immunofluorescence staining of tau pS416 (green), tauC3 (red), and nuclei (Hoechst 33342, blue) from the hippocampal CA1/CA2 region of human patient brains across increasing spectrum of Alzheimer’s Disease Neuropathological Change (ADNC) scoring. Scale bars = 50 µM; insets = 10 µM. B Quantification of tau pS416 staining across ADNC score. Box plot bounds dictate 25th−75th percentile, whiskers define sample minima and maxima, and line shows sample median. Analysis was performed on four unique patient samples for each score. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc analysis. C Quantification of tauC3 staining across ADNC score. Box plot bounds dictate 25th−75th percentile, whiskers define sample minima and maxima, and line shows sample median. Analysis was performed on four unique patient samples for each score. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc analysis. D Two-sided Pearson’s analysis showing correlation of increasing tau pS416 and tauC3 across ADNC score.
