Fig. 2: Proposed biosynthetic pathway of penicillic acid (1).
From: Substrate specificity of a branch of aromatic dioxygenases determined by three distinct motifs
a Penicillic acid (1) biosynthetic gene cluster (BGC) in A. westerdijkiae. NR-PKS, nonreducing polyketide synthase; FMO, flavin-containing monooxygenase; OMeT, O-methyltransferase; SDR, short chain reductase; MFS, major facilitator superfamily; TF, transcription factor. b Product profiles of wild-type and mutants (ΔpaA, ΔpaD, and ΔpaE) of A. westerdijkiae. c In vitro assays of PaB and PaC with 2. d In vitro assays of PaD with 4 or 8. e LC-MS analysis of product profiles of compound 4 fed to S. cerevisiae expressing paD and paE. S. cerevisiae containing empty pXW55 and pXW06 vectors was used as a control. f Proposed biosynthetic pathway of 1.
