Fig. 4: Crystal structure analysis of PaD-4 complex.
From: Substrate specificity of a branch of aromatic dioxygenases determined by three distinct motifs
a Overall structure of PaD-4 complex (PDB: 8Z4R). Loops 1-3 are marked in gold. b The active site view of PaD-4 complex. The electron density 2Fo-Fc maps of substrate 4 in PaD are shown with a gray-colored mesh, and contoured at 1 σ. c Relative activities of PaD mutants located on the loops 1-3. The yield of 9 is quantified, defining the activity of wild-type PaD at 100%. Three biological parallel (n = 3) replicates are performed and presented as triangular points. The error bars are presented as standard deviation (SD). ND: not detected. Source data are provided as a Source Data file. d Comparison of the active-site shape and the substrate-binding mode in PaD and BTG13. The BTG13 complex structure was stimulated by Discovery Studio Client v19.1.0. e Proposed catalytic mechanism for PaD.
