Fig. 2: Inhibition of FOS alleviates the cytotoxicity of A. baumannii infection.

a Schematic representation of the FOS inhibition experiments. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). b, c Lactate dehydrogenase (LDH) release assay in A549 (24 hpi), MRC-5, BJ, HaCaT (12 hpi), and RAW264.7 (24 hpi) cells infected with A. baumannii and treated with the FOS inhibitors T5224 (100 μM). d, e Quantification of BIRC3, BCL3, CDK5, BNIP3, and BIM mRNA levels by qRT-PCR in A549 cells infected with A. baumannii (3 hpi) with or without T5224 treatment (100 μM). n = 3 independent experiments. f Western blot analysis of A549 cells infected with A. baumannii (6 hpi) with or without T5224 treatment (100 μM); * indicates nonspecific bands. The experiment was repeated three times with similar results obtained. g The mouse infection challenge model with T5224 treatment. Wild-type BALB/c mice were administrated orally with T5224 (250 mg/kg) or vehicle before and after injection i.p. with 3 × 10³ CFU of A. baumannii clinical isolate FDA-CDC AR-BANK#0280 (#0280). The animals were euthanized 8 hpi. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). h Western blot analysis of tissues harvested from the mouse infection model described in (h). Three pairs of infected animals were analyzed, and similar results were obtained. b, c n = 4. Cells were seeded in four different wells per group, Treatment and measurement were performed independently for each well. Experiments were repeated independently three times and similar results were obtained. b–e Data are presented as mean values ±SD, P-values by unpaired two-tailed t-test. See also Supplementary Fig. 2. Source data are provided in the Source Data file.