Fig. 6: PRDM16, NKX2-1, and PRDM3 bind to shared sites throughout the genome and at promoters.

a, b Genomic distributions of all called peaks from a representative PRDM16 binding experiment, (n = 2 replicates across two independent experiments)(a) and a representative NKX2-1 binding experiment, (n = 3 replicates across two independent experiments)(b) are shown. c Genomic distribution of the overlap of PRDM16 and NKX2-1 bound peaks that are marked by H3K4me3. d, e HOMER motif enrichment for all called peaks across the genome of a representative PRDM16 CUT&RUN (d) and a NKX2-1 CUT&RUN (e) experiment, p-value < 0.05, determined by binomial test comparing frequency of a given motif under a set of peaks compared to a randomly generated background. f, g Selected CUT&RUN gene tracks from H3K4me3, PRDM16, PRDM3, and NKX2-1 CUT&RUN experiments are visualized with the UCSC Genome Browser. AT1 and AT2 cell associated peaks were obtained from Little, et al.¹¹. ENCODE cCRE peaks are annotated from the ENCODE database of cis-regulatory elements. Binding is seen in both AT2 cell-associated genes (f) and AT1 cell-associated genes (g).