Fig. 8: AT2-specific PRDM3/16 deletion leads to lineage infidelity during alveolar epithelial specification.

a Dams were treated with tamoxifen at E12.5 and E13.5 to generate Prdm3/16^{SftpcCreER+/Δ}; Rosa26^lsl-tdTomato (Control) and Prdm3/16^{SftpcCreERΔ/Δ}; Rosa26^lsl-tdTomato (KO) fetuses which were harvested at E18.5 for lineage analysis. Control animals (b–i) demonstrated a majority of cells in the Sftpc-lineage (Sftpc^Lineage) were marked with only SFTPC protein by IHC (white arrowheads), while KO animals (j–q) showed significant decreases in SFTPC-only cells (r), with corresponding increases in Sftpc^Lineage cells expressing HOPX (s), either with concomitant SFTPC expression (t) (yellow arrowheads in l, m, p, q) or without (u)(green arrowheads in l, m, p, q). n = 6 control and n = 5 KO lungs. Significance was determined by unpaired 2-tailed t-Test. The graphs are min point to max point showing all points with the center as mean and the box is 25-75%. All scale bars are 50 μm.