Fig. 2: Loss of USP26 inhibits cell proliferation and promotes cell apoptosis in vitro. | Nature Communications

Fig. 2: Loss of USP26 inhibits cell proliferation and promotes cell apoptosis in vitro.

From: USP26 as a hepatitis B virus-induced deubiquitinase primes hepatocellular carcinogenesis by epigenetic remodeling

Fig. 2

Western blot analysis (a), MTT (b), colony formation (c) and EdU staining assays (d, e) of USP26-knockdown HCCLM3 cells with or without USP26 (shRNA-resistant USP26) restoration. Scale bar: 100 μm. Western blot analysis (f), MTT (g), colony formation (h) and EdU staining assays (i, j) of USP26-knockdown MHCC97H cells with or without USP26 (shRNA-resistant USP26) restoration. Scale bar: 100 μm. k–m. The apoptosis rate of MHCC97H cells as described in f was measured by flow cytometry after Annexin/V and PI dual staining. n Cleaved PARP (C-PARP) expression in MHCC97H cells as described in f was measured by western blotting. The red stars (*) in a, f and n indicate the positions of USP26. Each graph is presented as the mean ± SEM. Each blot data is representative of three independent experiments. (b, g) n = 4 independent experiments; (c, d, h, i, k) n = 3 independent experiments. Statistical significance was calculated by (b, g) two-way ANOVA; (c, e, h, j, l, m) one-way ANOVA. Source data are provided as a Source Data file.

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