Fig. 2: Expression of Lbx1, cMet, Nkx2.5 and MyoD in developing emu forelimb buds. | Nature Communications

Fig. 2: Expression of Lbx1, cMet, Nkx2.5 and MyoD in developing emu forelimb buds.

From: Immobilization secondary to cell death of muscle precursors with a dual transcriptional signature contributes to the emu wing skeletal pattern

Fig. 2

a Expression of Lbx1, cMet and Nkx2.5 (arrowheads) in forelimb fields (FL) of emu embryos (stage 18 Lbx1 (n = 6), cMet (n = 2), Nkx2.5 (n = 4); stage 19 Nkx2.5 (n = 2)). Scale bars, 500 μm. b Expression of Lbx1, cMet, Nkx2.5 and MyoD (arrowheads) in serial sections of emu forelimb buds at mid- (mid. FL) or posterior level (post. FL). A dotted circle indicates Nkx2.5 negative region. Panels of stages 19 and 23 sections were flipped horizontally. (stage 19 Lbx1 (n = 6), cMet (n = 4), Nkx2.5 (n = 6); stage 23 cMet (n = 2), Nkx2.5 (n = 2); stage 25 Lbx1 (n = 3), Nkx2.5 (n = 3), MyoD (n = 2)). dm, dermomyotome; D, dorsal; V, ventral. Scale bars, 50 μm. c, c’, c” Distribution of cMet and Nkx2.5 transcripts in developing emu forelimb buds at stage 23 (n = 4). Arrows indicate the cMet transcripts in muscle precursors derived from the dermomyotome. Arrowheads indicate co-locaized transcripts of cMet and Nkx2.5. d, d’ Distribution of cMet, Nkx2.5 and MyoD transcripts in emu forelimb buds at stage 25 (n = 3). Note that transcripts of cMet and Nkx2.5 (arrowheads) are present in cell clusters, in which MyoD expression is also detected (arrows). White cells are blood cells (asterisks), not stained cells.

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