Fig. 7: Enhancing sPG synthesis promotes Z ring condensation and stability in C. crescentus.
From: The divisome is a self-enhancing machine in Escherichia coli and Caulobacter crescentus
a Cell length distribution of WT and ΔzapA C. crescentus cells with or without superfission mutations (3 biological replicates). Strains were grown in PYE medium at 30 °C in exponential phase, Number of cells analyzed (WT: 638; ΔzapA: 627; ΔzapA ftsW*: 770; ΔzapA ftsW** ftsI*: 606). Data are presented as mean values +/- s.d.. ****P  <  0.0001, two-tailed Student’s t test. b Representative images of Z rings in WT and ΔzapA C. crescentus cells with or without superfission mutations (3 biological replicates). Strains were grown in PYE medium at 30 °C with xylose to induce expression of FtsZ-mNeonGreen. Scale bar 2 μm. c-d Z ring width distribution (c) and percentage of condensed and decondensed Z rings (d) in WT and ΔzapA C. crescentus cells with or without superfission mutations (3 biological replicates). Number of rings analyzed (WT: 478; ΔzapA: 519; ΔzapA ftsW*: 482; ΔzapA ftsW** ftsI*: 364). The box plots in (c) show the 25th and 75th percentiles as the box limits, with the mean at the center, whiskers extend from -s.d. to +s.d. of the mean. e Representative time-lapse series of Z ring dynamics in WT and Δzap C. crescentus cells from 3 biological replicates. Samples were spotted onto PYE-agarose pad and imaged for 10 min with an interval of 1 min. Scale bar 2 μm. Source data are provided as a Source Data file.
