Fig. 4: Srf is required for the sustained proliferation of CD8+ T cells. | Nature Communications

Fig. 4: Srf is required for the sustained proliferation of CD8+ T cells.

From: IL-2 delivery to CD8+ T cells during infection requires MRTF/SRF-dependent gene expression and cytoskeletal dynamics

Fig. 4

A Frequencies of adoptively co-transferred (1 × 106 cells) OT-I WT TamCre and OT-I Srf−/− TamCre in spleens from uninfected animals compared with day 1 to day 4 post LM-OVA infection (left), or at days 3 and 4 post LM-OVA infection (right). Data show mean values ± SEM; datapoints represent individual mice, n = 3 mice (left) n = 4 mice (right). Representative of \(\ge\)2 experiments. Paired two-tailed t test is used for p value determination. B Frequencies of adoptively co-transferred (1 × 106 cells) OT-I WT TamCre and OT-I Mrtfab−/− TamCre in spleens from uninfected animals compared with day 2 to day 3 post LM-OVA infection (left), or at day 4 post LM-OVA infection (right). Data show mean values ± SEM; datapoints represent individual mice, n = 4 mice. Representative of \(\ge\)2 experiments. Paired two-tailed t test. C Upper panels, representative flow cytometry profiles of BrdU incorporation in OT-I WT and OT-I Srf−/− in spleen labelled from days 1–3 and days 6-8 post-infection. Lower panels, representative flow cytometry profiles of caspase-3 activity in OT-I WT and OT-I Srf−/− cells at day 3 and day 8 post-infection, Quantitation is as (A). D Quantification of CFSE-labelling profiles of MACS-purified CD8 cells, either resting or activated with plate-bound anti-CD3/CD28 (5 μg/ml) for the indicated times. A representative of three independent experiments is shown; datapoints show mean of triplicate determinations ± SEM. For CFSE profiles see Fig. S4C. Unpaired two-tailed t test is used for p value determination. E Plate-bound anti-CD3/CD28 TCR-induced transcription of IL-2 mRNA in sorted OT-I lymph node cells. A representative of three independent experiments is shown. Datapoints show mean of triplicate determinations ± SEM. F IL-2 production measured by ELISA in sorted CD8+ T lymph node cells following activation by plate-bound anti-CD3/anti-CD28. Data are triplicate assays (±SEM) from one of two independent experiments. Source data are provided as a Source Data file.

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