Fig. 5: Constitutive SKN-1 Activation results in serotonin limitations.
From: Serotonin deficiency from constitutive SKN-1 activation drives pathogen apathy
A Heat map of neuronal enrichment genes differentially expressed upon PA14 exposure between WT and skn-1gf animals, in comparison to OP50. B Representation of serotonin signaling pathways, including biosynthesis, uptake, and reuptake. C Selective representation of differentially expressed genes involved in serotonin signaling between WT and skn-1gf upon PA14 and OP50 exposure. D ELISA measurement of serotonin levels in whole animal extracts from WT and skn-1gf. E skn-1gf animals display enhanced sensitivity to treatment with the selective serotonin reuptake inhibitor fluoxetine. F Serotonin supplementation alleviates the apathy response of skn-1gf animals but only with continuous treatment from the earliest larval stage (G). Each of the food choice assay comprised of N ≥ 3 (with ≥ 150 worms per biological replicate per strain/condition) and analyzed via two-way ANOVA test; **(p < 0.01) ***(p < 0.001) ****(p < 0.0001). For serotonin ELISA (N = 2; n = 2) were analyzed via a two-tailed/sided paired t test; **(p < 0.01) ***(p < 0.001) ****(p < 0.0001). Individual “p” value numbers for the comparisons within each graph are placed above the respective comparison bars in the graph. All experiments were conducted at 25 °C. RNAseq data is available at the NIH (GEO) Gene Expression Omnibus (GSE251677). Source data for all Genotype-specific transcriptional responses are provided as a Source Data file 3. Source data for all behavioral responses are provided as a Source Data file 1.
