Fig. 6: An overview of the metabolic pathways impacted by statistically significant genes underlying clinical symptoms.

All genes annotated were found to have reduced the flux span through the metabolic system when knocked out. Genes coloured in pink and purple carried mutations or are accessory genes associated to the clinical symptom, respectively, and connected metabolic pathways (labelled in blue). The genes coloured in purple were also found as statistically significant in differentiating the BD-2 and BD-1.2 lineages (see previous sections). All 3D protein structures were generated in Alphafold123 under a Creative Commons Attribution 4.0 license (CC-BY 4.0), no changes were made.