Fig. 7: 3D protein structure analysis of FabV allelic variants underlying BD-1.2 and BD-2 lineage evolution and clinical symptoms.

A Violin plot indicating the distribution of the diarrhoea duration score (0: no diarrhoea, 1: <1day, 2: 1–3 days, 3: 4–6 days and 4: 7–9 days) for the isolates containing either Pro149 (P) or His149 (H). Statistical significance was tested with a two-sided Mann Whitney U test, p-value is shown. B Violin plot indicating the distribution of the number of stools score (0: <3 times, 1: 3–5 times; 2: 6–10 times; 3: 11–15 times; 4: 16–20 times; 5: 21+ times) for the isolates containing either Pro149 (P) or His149 (H). Statistical significance was tested with a two-sided Mann Whitney U test, p-value is shown. C The bar graph displays the number of isolates in the two BD lineages associated with Pro149 (P) and His149 (H). D 3D structures of FabV (AlphaFold) with Pro149 and coloured by functional domains. Amino acid residues (Lys148, Ser151, and Trp159) interacting with Pro149 (green) are shown in sticks models. E 3D structures of FabV (AlphaFold) with His149 and coloured by functional domains. Amino acid residues (Lys148, Arg 150, Ser151, and Trp159) interacting with His149 (purple) are shown in sticks models.