Fig. 4: siLP-derived ST2^–KLRG1⁺IL-17RB⁺ ILC2s mediated asthmatic responses independent of adaptive immune cells.

A WT or Rag1^–/– mice were stimulated with HDM, and anti-KLRG-1 mAb or isotype IgG were administered intraperitoneally as indicated. B The percentage and the number of CD45⁺lineage^–CD90.2⁺NK1.1^–NKp46^–GATA3⁺ST2^–KLRG1⁺IL-17RB⁺ ILC2s in lungs were analyzed on day 24. C Pulmonary IL-13 was detected by ELISA. D Airway resistance was measured under stimulation of methacholine. E Pulmonary histological changes were observed by hematoxylin-eosin staining. Magnification: ×200, scale bar = 100 μm. F Rag1^–/– mice were stimulated with HDM, and CD45⁺lineage^–CD90.2⁺ST2^–KLRG1⁺IL-17RB⁺ ILC2s were sorted from siLP on D30. In vitro culturing cells were treated with anti-KLRG1 mAb or isotype control, and then adoptively transferred into untreated ILC2 depleted (anti-CD90.2 mAb or isotype control) Rag1^–/– mice via tail vein (5 × 10⁵ cells/mouse). Recipient mice were intranasally treated (i.n.) with 25 μg HDM or 500 ng recombinant IL-25 for 3 consecutive days. G The percentage and the number of CD45⁺lineage^–CD90.2⁺NK1.1^–NKp46^–GATA3⁺ST2^–KLRG1⁺IL-17RB⁺ ILC2s in lungs. H, I IL-13 level in lungs homogenates H and airway resistance I was measured. J Pulmonary histological changes were analyzed. Magnification: ×200, scale bar = 100 μm. Results are representative of three independent experiments. Data was shown as Means ± SD. n = 6 in all panels except for n = 3 in D and I. Statistical comparison was conducted using unpaired, two-sided Welch’s t test, except in (D) and (I) using unpaired two-way ANOVA with the Bonferroni posttest to analyze dose response to methacholine. p values are shown on the graphs. Source data are provided as a Source Data file. Fig. 4F Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en.