Fig. 6: Virtual birefringence imaging with a shifted angle. | Nature Communications

Fig. 6: Virtual birefringence imaging with a shifted angle.

From: Virtual birefringence imaging and histological staining of amyloid deposits in label-free tissue using autofluorescence microscopy and deep learning

Fig. 6

a The virtual staining neural network is trained to simultaneously generate brightfield, cross-polarized birefringence, and birefringence images with a shifted angle using three digital staining matrices having pixel values of 1, −1 and 2, respectively. b–d Three separate fields-of-view are shown in three panels. The top row displays histochemical brightfield and polarization Congo red images. The second row presents virtually stained brightfield and cross-polarized birefringence images. The bottom row shows virtual polarization images with a shifted angle that features yellow-colored amyloid deposits.

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