Fig. 1: Merlin drives meningioma Wnt signaling.
From: MerlinS13 phosphorylation regulates meningioma Wnt signaling and magnetic resonance imaging features
a Immunoblots for FLAG (Merlin) or GAPDH in CH-157MN meningioma xenografts with or without 24 h of doxycycline-inducible Merlin rescue (200 μg/ml). Representative of four experimental repeats. b CH-157MN xenograft measurements in NU/NU mice with (n = 12) or without (n = 12) doxycycline-inducible Merlin rescue as in (a). c Kaplan-Meier survival curve for CH-157MN xenograft overall survival in NU/NU mice as in (b) (log-rank test). d Uniform manifold approximation and projection (UMAP) of single-cell RNA sequencing transcriptomes of 40,765 CH-157 cells from 12 xenografts (n = 5 Merlin-deficient CH-157MN xenografts, n = 7 CH-157MN xenografts with Merlin rescue) as in (a–c) colored by assignments from Louvain clustering. e Quantification of single-cell types from (d) that were differentially enriched in Merlin-deficient compared to Merlin rescue xenografts. f Feature plots for the MSigDB Hallmark Wnt target gene expression signature in single-cell transcriptomes from Merlin-deficient (n = 5) compared to Merlin rescue xenografts (n = 7). g TOP-Flash Tcf/Lef luciferase reporter assay in M10GdCas9-KRAB meningioma cells expressing non-targeted control sgRNAs (sgNTC) or sgRNAs suppressing NF2 (sgNF2) with or without 24 h of Wnt3a treatment (100 ng/μl). Representative of three experimental repeats. Lines represent means, and error bars represent standard error of the means. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.0001 (Student’s t test, one sided). Source data are provided as a Source Data file.
