Fig. 1: CD49b expression in the HSC compartment is conserved in aging.

a FACS profiles and gating strategy of the phenotypic HSC compartment (Lineage^–Sca-1⁺c-Kit⁺ (LSK) CD48^–CD34^–CD150^hi), with further separation using CD49b, in juvenile, adult, and old mice. Frequency of parent gates are shown. See Supplementary Fig. 1a for the full gating strategy. b Total frequency of CD49b^– and CD49b⁺ HSC subsets in juvenile (n = 13 mice, 3 experiments), adult (n = 12 mice, 6 experiments), and old (n = 6 mice, 6 experiments) mice. c Total numbers of CD49b^– and CD49b⁺ HSC subsets in juvenile (n = 13 mice, 3 experiments), adult (n = 12 mice, 6 experiments), and old (n = 6 mice, 6 experiments) mice. d Frequency of CD49b^– and CD49b⁺ HSCs in G0 (left) and G1 (right) of juvenile (n = 9 mice, 3 experiments), adult (n = 15 mice, 6 experiments), and old (n = 8 mice, 5 experiments) mice. e Frequency of cell divisions from cultured single cells of CD49b^– (left) and CD49b⁺ (right) HSCs at days 1–3 from juvenile (n = 7 mice, 3 experiments, n_CD49b^– = 351 cells, n_CD49b⁺ = 258 cells), adult (n = 4 mice, 3 experiments, n_CD49b^– = 149 cells, n_CD49b⁺ = 146 cells), and old (n = 6 mice, 4 experiments, n_CD49b^– = 599 cells, n_CD49b⁺ = 539 cells) mice. f Frequency of BrdU⁺ CD49b^– and CD49b⁺ HSCs from juvenile (n = 11 mice, 3 experiments), adult (n = 10 mice, 3 experiments), and old (n = 8 mice, 3 experiments) mice. Mean ± s.d. is shown. The statistical analyses were performed two-sided with one-way ANOVA with Tukey’s multiple comparison test in the CD49b^– subset in b–d and CD49b⁺ G0 in d, two-way repeated measures ANOVA with Tukey’s multiple comparison test in e, Kruskal–Wallis with Dunn’s multiple comparison test in the CD49b⁺ subset in b and c, CD49b⁺ G1 in d, and in f. J juvenile, A adult, O old. See also Supplementary Fig. 1. Source data are provided as a Source Data file.