Fig. 2: The lineage repopulation patterns of multipotent CD49b subsets change with age. | Nature Communications

Fig. 2: The lineage repopulation patterns of multipotent CD49b subsets change with age.

From: Aging is associated with functional and molecular changes in distinct hematopoietic stem cell subsets

Fig. 2

a Total frequency of clones containing B cells (left), or only myeloid cells (right) from CD49b subsets in juvenile (nCD49b– = 6 mice, nCD49b+ = 7 mice, 3 experiments), adult (nCD49b– = 5 mice, nCD49b+ = 6 mice, 3 experiments), and old (nCD49b– = 7 mice, nCD49b+ = 7 mice, 4 experiments) mice. b Megakaryocyte potential of CD49b subsets from juvenile (nCD49b– = 5 mice, nCD49b+ = 5 mice, 3 experiments), adult (nCD49b– = 7 mice, nCD49b+ = 7 mice, 6 experiments), and old (nCD49b– = 4 mice, nCD49b+ = 4 mice, 4 experiments) mice. c Donor leukocyte contribution in the PB of transplanted mice (nJCD49b– = 14 mice, nJCD49b+ = 24 mice, nACD49b– = 5 mice, nACD49b+ = 10 mice, nOCD49b– = 20 mice, nOCD49b+ = 18 mice). d Donor-derived lineage contribution in the PB of transplanted mice (nJCD49b– = 14 mice, nJCD49b+ = 24 mice, nACD49b– = 5 mice, nACD49b+ = 10 mice, nOCD49b– = 20 mice (11 for P-E), and nOCD49b+ = 18 mice (15 for P-E)). e Relative lineage contribution within donor leukocytes and donor chimerism (CD45.2) in the PB 5–6 months post-transplantation from d. f Proportion of lineage distribution patterns from e, using adult L/M ratio (nJCD49b– = 12 mice, nJCD49b+ = 20 mice, nACD49b– = 5 mice, nACD49b+ = 10 mice, nOCD49b– = 20 mice, nOCD49b+ = 18 mice). g L/M ratio in PB of mice transplanted with old CD49b subsets (nCD49b– = 20 mice, nCD49b+ = 18 mice) 5–6 months post-transplantation. The ranges for L-bi, Bal, and M-bi based on old L/M ratio are indicated. h Relative lineage contribution to myeloid (M) and lymphoid cells (L: B, T, and NK cells) in the BM of transplanted mice (nJCD49b– = 13 mice, nJCD49b+ = 19 mice, nOCD49b– = 20 mice, nOCD49b+ = 18 mice). Mean ± s.d. is shown. Statistical analyses were performed two-sided, with Kruskal–Wallis with Dunn’s multiple comparison test in a and CD49b– in b, one-way ANOVA with Tukey’s multiple comparison test in CD49b+ in b, Mann–Whitney test in c, and Wilcoxon signed-rank test in h, except in JCD49b–, where paired t-test was done. J juvenile, A adult, O old, PB peripheral blood, BM bone marrow, L-bi lymphoid-biased, Bal balanced, M-bi myeloid-biased, L/M lymphoid to myeloid. See also Supplementary Figs. 2–4. Source data are provided as a Source Data file.

Back to article page