Fig. 3: The CD49b– HSC subset is the most durable subset regardless of age.
a Proportion of mice exhibiting myeloid repopulation in the peripheral blood and HSC (LSK CD48–Flt-3–CD150+) repopulation in the bone marrow (BM), 5–6 months after primary or secondary transplantation of juvenile CD49b– and CD49b+ HSCs (nCD49b–Primary = 15 mice, nCD49b+Primary = 23 mice, nCD49b–Secondary = 12 mice, nCD49b+Secondary = 8 mice). b Frequency of HSC repopulation in reconstituted mice after primary transplantation (nJCD49b– = 12 mice, nJCD49b+ = 7 mice, nOCD49b– = 20 mice, nOCD49b+ = 17 mice). c Proportion of mice exhibiting myeloid repopulation in the peripheral blood and HSC (LSK CD48–Flt-3–CD150+) repopulation in the BM, 5–6 months after primary or secondary transplantation of old CD49b– and CD49b+ HSCs (nCD49b–Primary = 20 mice, nCD49b+Primary = 18 mice, nCD49b–Secondary = 17 mice, nCD49b+Secondary = 10 mice). d Total donor leukocyte contribution in the PB of mice secondary transplanted with CD49b– and CD49b+ HSC subsets from juvenile, adult, and old mice (nJCD49b– = 12 mice, nJCD49b+ = 8 mice, nOCD49b– = 17 mice, nOCD49b+ = 10 mice). Mean ± s.d. is shown. The statistical analyses were performed two-sided, with Fisher´s exact test in a and c, Mann–Whitney test in b, and Šídák’s multiple comparisons test after adjusting for repeated measures in d. J juvenile, O old, PB peripheral blood. See also Supplementary Fig. 4. Source data are provided as a Source Data file.
