Fig. 5: Hippo pathway inhibition affects atrial cardiomyocyte elongation.
From: Distinct mechanisms regulate ventricular and atrial chamber wall formation
a–d 3D airyscan images of atria from 124 hpf larvae treated from 72 to 124 hpf with DMSO (a, c), K-975 (b), or IWR-1(d); CM membranes shown in cyan (myl7:EGFP-Hsa.HRAS) and CM nuclei in yellow (myl7:H2B-mScarlet). e, g 3D quantification of atrial CM shape in 124 hpf larvae treated from 72 to 124 hpf with DMSO, K-975, or IWR-1 (n = 41 atrial CMs from 4 DMSO hearts, n = 36 atrial CMs from K-975 hearts, n = 52 atrial CMs from 5 DMSO hearts, and n = 58 atrial CMs from 5 IWR-1 hearts; each data point represents one atrial CM; average of 10 CMs per heart two-tailed Mann-Whitney test (e); two-tailed unpaired Student’s t-test p-value = 0.48*10-4 (g)). f–f”, h–h”, Atrial CM angle measured in 124 hpf larvae treated from 72 to 124 hpf with DMSO, K-975, or IWR-1 (n = n = 41 atrial CMs from 4 DMSO hearts, n = 36 atrial CMs from 4 K-975 hearts, n = 49 atrial CMs from 5 DMSO hearts, and n = 59 atrial CMs from 5 IWR-1 hearts; each data point represents one atrial CM; average of 10 CMs per heart; two-tailed Mann-Whitney tests). Error bars are mean ± SD.
