Fig. 1: Drosophila germ granules have a biphasic organization.
From: Spatial organization of translation and translational repression in two phases of germ granules
a STED imaging of germ granule main components. Immunostaining of UASp-GFP-Aub; nos-Gal4 or vasa-GFPKI embryos with anti-Aub (magenta), anti-Osk (magenta), anti-Tud (magenta), and anti-GFP (green) to visualize GFP-Aub or Vasa-GFP. Fluorescence intensity (right) was measured along the path marked with a white dashed line and intensity profiles of each channel are shown on the graph. b 3D-OMX imaging of UASp-GFP-Aub; nos-Gal4 (left), GFP-TudKI (middle), Vasa-GFPKI (right) in which GFP was directly recorded without antibody staining. c 3D-OMX imaging of UASp-GFP-Aub/ vasa-tdTomKI; nos-Gal4/+ embryos in which GFP and tdTomato were directly recorded without antibody staining. d, e Measurement of germ granule size (d) and outer phase size (e) from wild-type embryos immunostained with Osk antibody and imaged using STED microscopy. Scale bars: 1 µm. Source data are provided as a Source Data file.
