Fig. 3: Localization of translation initiation factors and translational repressors in the germ plasm.
From: Spatial organization of translation and translational repression in two phases of germ granules
a, b Localization of translation initiation factors. STED imaging of immunostaining of UASp-GFP-Aub/UASp-HA-eIF3d; nos-Gal4/+ (a) and UASp-GFP-Aub; nos-Gal4 (b) embryos with anti-GFP (magenta) to detect Aub, anti-HA (green) to detect eIF3d (a) and anti-PABP (green) (b). c–i Localization of translational repressors. STED imaging of immunostaining of UASp-GFP-Aub; nos-Gal4, GFP-belle, and wild-type embryos with antibodies to reveal the indicated proteins. Germ granule markers are in magenta and translational repressors are in green. A larger germ granule exemplifying those present at later stages in primordial germ cells (PGC) is shown in (f). j Quantification of colocalization of GFP-Aub or Osk with the indicated proteins using PCC(Costes) from images as in (a–e and g–i). Black circles represent the mean and error bars represent SEM. The number of embryos is indicated (n). k Quantification of the distance between Smaug (Smg) foci and the edge of germ granules marked with GFP-Aub from images as in (c). Vertical bars represent the mean and SD. **p < 0.01 using χ2 test. p = 0.0033. Scale bars: 1 µm. Source data are provided as a Source Data file.
